rabbit anti mouse scd1  (Cell Signaling Technology Inc)

Journal: PLoS ONE

Article Title: The PPAR pan-agonist tetradecylthioacetic acid promotes redistribution of plasma cholesterol towards large HDL

doi: 10.1371/journal.pone.0229322

Figure Lengend Snippet: a. mRNA expression of Scd1 was analyzed on individual samples (n = 6) with S1 of one individual in the HFD control group used as a calibrator. Data in bar plots are shown as mean ± SEM, in HFD as dark grey bars with individual values in light grey circles and in HFD+TTA as light grey bars with individual values in dark grey circles and student´s unpaired t-test (two tailed) was performed, * = p<0.05 and ** = p<0.01. b. Immunohistochemistry of the middle part of the intestine using an antibody against SCD1. Left panel, HFD control and right panel, HFD+TTA.

Article Snippet: Consecutive sections were incubated overnight with a polyclonal guinea pig anti-mouse Perilipin2 (Progen, GP40) in 1:2000 dilution, and monoclonal rabbit anti-mouse SCD1 (Cell Signaling #2794) in dilution 1:100.

Techniques: Expressing, Control, Two Tailed Test, Immunohistochemistry

Journal: Molecular medicine reports

Article Title: Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes.

doi: 10.3892/mmr.2017.6473

Figure Lengend Snippet: Figure 3. Effects of THP on SREBP1 target protein and gene expressions. Confluent cells were treated with various concentrations (0, 10, 20 or 40 µM) of THP from day 0 to 4. On day 8, completely differentiated cells were lysed to extract total protein. (A) Protein extracts were prepared and subjected to western blot analysis using SREBP1c, FAS and SCD1, (B) On day 8, completely differentiated cells were used to extract total mRNA. The expression of adipogenesis‑associated genes SREBP1c, FAS, SCD1 and GPAT were measured by reverse transcription‑quantitative polymerase chain reaction. Data are represented as the mean ± standard error of the mean (n=3). **P<0.01 vs. DM control. THP, tetrahydropalmatine; DM, differentiation medium; SREBP1c, sterol regulatory element‑binding protein 1; FAS, fatty acid synthase; SCD1, stearoyl‑CoA desaturase‑1; GPAT, glycerol‑3‑phosphate O‑acyltransferase.

Article Snippet: Rabbit anti-mouse polyclonal FAS (3180; 1:2,000), rabbit anti-mouse monoclonal SCD1 (2794; 1:2,000), rabbit anti-mouse polyclonal PPARγ (2435; 1:2,000), rabbit anti-mouse polyclonal C/EBPα (2295; 1:2,000), rabbit anti-mouse polyclonal phospho (p)-AMPKα (2531L; 1:2,000), rabbit anti-mouse polyclonal AMPKα (2532S; 1:2,000), rabbit anti-mouse polyclonal p-ACC (3661L; 1:2,000) and rabbit anti-mouse polyclonal ACC (3662; 1:2,000) antibodies were from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Western Blot, Expressing, Polymerase Chain Reaction, Control

Journal: PLoS ONE

Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: List of primer sequences used for RT-qPCR.

Article Snippet: Immunohistochemistry was performed using the following primary antibodies: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against mouse SCD1 (#2794, Cell Signaling), and rat monoclonal antibody against mouse UMOD (clone 774056; R&D Systems).

Techniques:

Journal: PLoS ONE

Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: ( A ) SCD1 was detected in the cytoplasmic compartment selectively of proximal tubular cells (in the straight S3 segment). In the kidney of the homozygous Umod C93F mutant mouse, SCD1 appeared to be abundant in a larger fraction of proximal tubular cells compared to the kidney of the wild-type mouse, and the average staining intensity of SCD1 positive cells appeared to be more prominent. Age of mice analyzed: four months. Umod wt : wild-type mouse; Umod C93F : homozygous Umod C93F mutant mouse. Uromodulin immunohistochemistry enabled identification of TALH segments. Proximal tubule segment are morphologically characterized by luminal microvilli. Chromogen: DAB for SCD1, Vector RED for UMOD; nuclear staining: hemalum. ( B ) Protein abundance of SCD1 in whole kidney lysate of homozygous Umod mutant mice of both lines was increased compared to wild-type mice. Signal intensities of SCD1 were corrected for GAPDH signal intensities of the same PVDF-membrane. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. One-way-ANOVA with Tukey's Multiple Comparison Post hoc Test: p vs. wild-type, **, p <0.01; ***, p<0.001. Age of mice analyzed: four months.

Article Snippet: Immunohistochemistry was performed using the following primary antibodies: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against mouse SCD1 (#2794, Cell Signaling), and rat monoclonal antibody against mouse UMOD (clone 774056; R&D Systems).

Techniques: Mutagenesis, Staining, Immunohistochemistry, Plasmid Preparation, Quantitative Proteomics, Membrane, Comparison

Journal: PLoS ONE

Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: Increased Scd1 transcript abundance in kidneys of homozygous Slc12a1 I299T mutant mice compared to the kidneys of littermate controls were detected by RT-qPCR analysis. Data are shown as scatter dot plot with mean (n = 5 per group). Age of mice analyzed: three months. Student's t test: p vs. wild-type, *, p <0.05.

Article Snippet: Immunohistochemistry was performed using the following primary antibodies: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against mouse SCD1 (#2794, Cell Signaling), and rat monoclonal antibody against mouse UMOD (clone 774056; R&D Systems).

Techniques: Mutagenesis, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: List of primer sequences used for RT-qPCR.

Article Snippet: The following primary antibodies were used: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against GAPDH (#2118, Cell Signaling), rabbit polyclonal antibody against mouse SCD1 (#2438, Cell Signaling).

Techniques:

Journal: PLoS ONE

Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: ( A ) SCD1 was detected in the cytoplasmic compartment selectively of proximal tubular cells (in the straight S3 segment). In the kidney of the homozygous Umod C93F mutant mouse, SCD1 appeared to be abundant in a larger fraction of proximal tubular cells compared to the kidney of the wild-type mouse, and the average staining intensity of SCD1 positive cells appeared to be more prominent. Age of mice analyzed: four months. Umod wt : wild-type mouse; Umod C93F : homozygous Umod C93F mutant mouse. Uromodulin immunohistochemistry enabled identification of TALH segments. Proximal tubule segment are morphologically characterized by luminal microvilli. Chromogen: DAB for SCD1, Vector RED for UMOD; nuclear staining: hemalum. ( B ) Protein abundance of SCD1 in whole kidney lysate of homozygous Umod mutant mice of both lines was increased compared to wild-type mice. Signal intensities of SCD1 were corrected for GAPDH signal intensities of the same PVDF-membrane. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. One-way-ANOVA with Tukey's Multiple Comparison Post hoc Test: p vs. wild-type, **, p <0.01; ***, p<0.001. Age of mice analyzed: four months.

Article Snippet: The following primary antibodies were used: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against GAPDH (#2118, Cell Signaling), rabbit polyclonal antibody against mouse SCD1 (#2438, Cell Signaling).

Techniques: Mutagenesis, Staining, Immunohistochemistry, Plasmid Preparation, Quantitative Proteomics, Membrane, Comparison

Journal: PLoS ONE

Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: Increased Scd1 transcript abundance in kidneys of homozygous Slc12a1 I299T mutant mice compared to the kidneys of littermate controls were detected by RT-qPCR analysis. Data are shown as scatter dot plot with mean (n = 5 per group). Age of mice analyzed: three months. Student's t test: p vs. wild-type, *, p <0.05.

Article Snippet: The following primary antibodies were used: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against GAPDH (#2118, Cell Signaling), rabbit polyclonal antibody against mouse SCD1 (#2438, Cell Signaling).

Techniques: Mutagenesis, Quantitative RT-PCR